Mechanical properties monitorization in bioreactor by ultrasound

Recent works describe the advances in the substitutive organ therapy [1, 2, 3], based on the development of the so-called bio-artificial organs (decellularized organs of human or animal origin), that benefit of their extracellular matrix, and whose cells are substituted through a recellularization process via primary cells that show ability to differentiate themselves in their interior. To validate the process, a culture of gingival fibroblasts in fibrin-agarose stroma is monitored in the bioreactor.
To standardize and optimize the process, it is necessary to control most of the parameters that may vary its effectiveness. We propose to monitor the changes that may suffer the matrix during the decellularization process using mechanical and optical parameters. To ensure the viability of this protocol, a bioreactor has been designed.

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